... Something to notice is usually that The existing LLM50 setup has a bigger vignetting region, or lesser imaging space, than the LLM36 by ~20%. The vignetting of the LLM50 could be diminished by eliminating the dichroic compartment and positioning the microscope objective closer on the tube lens [28], https://bio-fluorescence68631.ageeksblog.com/7139086/fascination-about-bio-fluorescence-scattering