Each Nikon triple band fluorescence set is optimized for use with DAPI and FITC (fluorescein isothiocyanate) in combination with both TRITC (tetramethylrhodamine isothiocyanate) or Texas Red probes. The relevant spectral regions akin to these fluorochrome combos range between violet excitation and blue emission to green excitation and pink emission. http://rivermifra.blogocial.com/Considerations-To-Know-About-Fluorescence-Filter-set-31432017